![]() You can use Add in the Bands menu in the top center menu bar to add boxes around individual bands to be quantified.Each band of interest should have one box around it. Add boxes to your bands (see below) and repeat for each channel. To select the bands of interest, select the 700 or 800 channel by clicking the X box next to the channel on the right side of the screen (with the vertical Display tab selected).Click Redraw Boundaries and drab the boundary to include all lanes (not ladder(s)).Adjust the Number of Lanes on the far left of the menu bar to fit your blot.With the Analysis tab selected, Select Western"' as a Type on the leftmost side of the menu bar.Using these tools will automatically create a new, adjusted image with a description of the changes from the original in the Images table. You can flip, rotate, and straighten the image to better align the lanes using Rotate or Flip' and Free Rotate in the top center menu.With the Image tab selected, select the image you want to analyze from the table at the bottom.Press Cancel on the image-adjuster pop-up. Re-scan for a high-resolution image by cliking Start on the top right menu bar (green circle with power button icon).Adjust the blue box to fit just around your blot.Add specifics to the image table (antibody, experiment, name of file: yy-mm-dd_nameofexperiment).After the scan is complete, a pop-up menu with come up to adjust the image. Adjust and re-scan with preview as needed. Use this image to capture all of the blot. Click Preview on the top right menu bar (blue circle with magnifying glass) to scan a preliminary, low resolution image.(Tip: Note the coordinates of your blot using the ruler-as above-and set blue box wider that blot initially). With the ' Acquire tab selected, click on the blue box in the large white-grid working space and drag edges to fit around your blot.Take mental note of where your blot is on the ruler on the scanning surface. Cover with clear rubber mat and roll with roller to remove any air pockets. Place blot (wet membrane if scanning for total protein, dry membrane if scanning finished blot) on scanner, protein side down.Click on or create (using your last name) your Work Area. Open ImageStudio (yellow icon on desktop).Clean glass scanning surface of the Odyssey gently with DI water and KimWipes to remove any dust or debris. ![]()
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